Evaluation of Oxford Nanopore MinION Sequencing for 16S rRNA Microbiome Characterization
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چکیده
منابع مشابه
Profiling bacterial communities by MinION sequencing of ribosomal operons
BACKGROUND An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRNA subunit), and a high-fidelity Taq polymerase with proofreading capabilities. Amplicons contained both ri...
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BACKGROUND Nanopore sequencing provides a rapid, cheap and portable real-time sequencing platform with the potential to revolutionize genomics. However, several applications are limited by relatively high single-read error rates (>10 %), including RNA-seq, haplotype sequencing and 16S sequencing. RESULTS We developed the Intramolecular-ligated Nanopore Consensus Sequencing (INC-Seq) as a stra...
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BACKGROUND The MinION™ nanopore sequencer was recently released to a community of alpha-testers for evaluation using a variety of sequencing applications. Recent reports have tested the ability of the MinION™ to act as a whole genome sequencer and have demonstrated that nanopore sequencing has tremendous potential utility. However, the current nanopore technology still has limitations with resp...
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BACKGROUND The miniaturised and portable DNA sequencer MinION™ has been released to the scientific community within the framework of an early access programme to evaluate its application for a wide variety of genetic approaches. This technology has demonstrated great potential, especially in genome-wide analyses. In this study, we tested the ability of the MinION™ system to perform amplicon seq...
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25 The miniaturized and portable DNA sequencer MinION has been released to the scientific community in the framework of an early access programme to evaluate its scope in a wide variety of genetic approaches. Although this technology is under constant development to completely deliver error-free and high quality reads, it has demonstrated a great potential especially in wide-genome analyses. In...
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